Column chromatography Study guides, Class notes & Summaries

BIOCHEMISTRY LAB REPORT 4 2023| PORTAGE LEARNING|WITH PROCEDURES, EXPLANATION AND FINAL RESULTS.HIGHLY VERIFIRD Experiment #: Lab 4 Title: Chromatography of molecules. Purpose: The goals for the student performing the experiment are 1. Explain the fundamentals of chromatography. 2. Demonstrate ion exchange and size- exclusion chromatography. 3. Separate a Real-World sample. The student should be able to use the techniques learned and be able to apply them to further use in the lab or ...

CHEM 212 Lab 2 Report Separation of Organic Compounds –Column Chromatography CHEM 212 Lab 2 Report Separation of Organic Compounds –Column Chromatography

CHEM 212 Lab 4 Report Separation of Organic Compounds – Column Chromatography CHEM 212 Lab 4 Report Separation of Organic Compounds – Column Chromatography

What are the benefits of having smaller particles in HPLC? - correct answers high efficiency, higher plate number, higher pressure, shorter run time, better resolution due to uniform flow (reduced A-term, multiple pathway) what is plate height? - correct answers length of column required for 1 equilibration of solute between mobile and stationary phases What are the drawbacks of having smaller particles in HPLC? - correct answers lower detection limit, resistance to solvent flow, increased...

You're using anion exchange chromatography to purify a small globular protein. After passing the mixture through the column, you find that your protein did not "stick" to the resin and flowed right through. What is a plausible reason for this to have occurred? Correct Answer-The pH of the solution is close to the isoelectric point. Oxygen binding that affects the cooperatively of hemoglobin is an example of heterotrophic regulation. Correct Answer-False You have determined that a leucin...

Increase N - correct answers 1) Increase length of column 2) Optimize flow rate (Ux) to minimize H 3) Smaller packing bead diameter, uniform packing (A-term) 4) Thinner SP layer (less C term) 5) Open Tubular Liquid Chromo (No A) (A: more sensitive & resolution, fit more plate (N) shorter analysis D: small capacity) Absorption Chromatography - correct answers SP: Solid MP: Liquid or Gas Solutes move at different rates according to the forces of attraction to the stationary phase Parti...

in size exclusion chromatography, you would Expect a. colored mol to elute off column prior to clear b. sm mol to elute off column faster than lg c. sm mol to bind to the column when the buffer pH is low d. sm mol to elute off column slower than lg mol - D. sm mol to elute off column slower than lg mol elevated serum pro and decreased albumin are associated with: a, glomerulonephritis b. cirrhosis. c. starvation/anorexia d. monoclonal gammopathy - d. monoclonal gammopathy beers law sta...

Albert's Chapter 8 Exam 115 Questions with Verified Answers WITTF: machine that sorts fluorescently labeled cells and nonlabeled cells into separate containers using a strong electric field - CORRECT ANSWER fluorescence-activated cell sorter How are antibodies used to fluorescently label cells? - CORRECT ANSWER fluorescent molecule attached to antibody, which binds to a very specific cell type How does a fluorescent-activated cell sorter work? - CORRECT ANSWER individual cells travel s...

Extraction - correct answers Transfer of a solute from one phase to another Eluent - correct answers Fluid entering the column Partition Chromatography - correct answers Separation based on solute dissolving into stationary liquid Ion-Exchange Chromatography - correct answers Separation based on charge, stationary phase is made of either negatively or positively charged beads Size Exclusion Chromatography - correct answers Separation based on molecular size, larger molecules elute fi...

BIOCHEMISTRY LAB REPORT 6 2023/2024| PORTAGE LEARNING|WITH PROCEDURES, EXPLANATION AND FINAL RESULTS.HIGHLY VERIFIED Column Chromatography and TLC of Spinach Group Experiment 3 – Individual Lab Report (Save as pdf and submit by NOON, 1 week after your experiment)