Some examples from this set of practice questions
1.
Which mechanism is associated with stochastic phase variation?
Answer: Slipped-strand mispairing.
2.
What is Roche 454 DNA sequencing?
Answer: PCR is carried out within a PCR reaction mix/ oil emulsion. DNA to be sequenced is fragmented, ligated to adapters and then separated into ssDNA.
3.
Which bacteria are intracellular pathogens?
Answer: Legionella pneumophila, Listeria monocytogenes, Salmonella typhimurium, Shigella dysenteriae
4.
Which statements are true about cell-mediated immunity? 1) It activates macrophages to kill intracellular pathogens. 2) It is impaired following bone marrow transplantation. 3) It plays a key role in the elimination of viruses. 4) It controls infection with Strongyloides stercoralis.
Answer: All four statements are true
5.
Successful bacterial colonisation of a mammalian host usually involves which factors?
Answer: Bacterial adhesion, avoidance of host defences, bacterial replication, and nutrient acquisition
6.
Which pathogen uses the integrin receptor for complement component C3bi, to invade macrophage?
Answer: Legionella pneumophila
7.
Bacterial effector proteins are delivered directly from the producing bacterium to the cytosol of eukaryotic target cells via which secretory mechanism?
Answer: Type 3 secretion system
8.
Which protein secretion systems are employed to secrete proteins across the inner (cytoplasmic) membrane into the periplasmic space?
Answer: The TAT secretion system and the Sec secretion system
9.
Which one of the following is true regarding the intracellular survival of pathogens? A. Coxiella burnetii quickly adapts to the phagolysosome. B. Mycobacteria use TACO to delay the maturation of their phagosome. C. Listeria monocytogenes escapes from the phagosome and propels itself to the perinuclear rER D. Legionella and Brucella spp. survive in a TGN-like compartment. E. Campylobacter induce the formation of a macropinosome using TTSS effectors.
Answer: B. Mycobacteria use TACO to delay the maturation of their phagosome.
10.
Which pairs of substrates are required for the biosynthesis of N-acylhomoserine lactones by LuxI homologues?
Answer: Acyl-ACP and S-adenosylmethionine
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